QIAGEN QuantiTect Rev.Transcription Kit

उत्पाद वर्णन

TheQIAGEN QuantiTect Reverse Transcription Kit (50)offers a streamlined solution for synthesizing high-quality cDNA from RNA templates, integrating genomic DNA (gDNA) removal and reverse transcription into a single, efficient workflow. Utilizing the unique gDNA Wipeout Buffer, the kit effectively eliminates contaminating gDNA, ensuring accurate downstream gene expression analysis. The combination of Quantiscript Reverse Transcriptase, optimized RT Buffer, and a proprietary RT Primer Mix facilitates robust cDNA synthesis from all regions of RNA transcripts, including challenging templates with high GC content or complex secondary structures. This kit is ideal for researchers aiming for sensitive and reproducible results in real-time two-step RT-PCR applications.

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• Integrated gDNA Removal: The gDNA Wipeout Buffer allows for effective elimination of genomic DNA contamination in just 2 minutes, negating the need for separate DNase treatments.

• Rapid cDNA Synthesis: Complete reverse transcription process in approximately 20 minutes, streamlining experimental workflows.

• High Sensitivity and Yield: Achieves high cDNA yields even from low-abundance transcripts, ensuring reliable quantification.

• Comprehensive Transcript Coverage: The RT Primer Mix, a blend of oligo-dT and random primers, enables cDNA synthesis from both 5' and 3' ends of RNA transcripts.

• Optimized for Real-Time PCR: The synthesized cDNA is compatible with real-time PCR assays, facilitating accurate gene expression analysis.

• Molecular Biology Research: Academic and industrial laboratories conducting gene expression and functional genomics studies.

• Clinical Diagnostics: Facilities performing RNA-based diagnostic assays requiring precise cDNA synthesis.

• Biotechnology and Pharmaceutical Development: Companies involved in drug discovery and development utilizing gene expression profiling.

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