QIAcuity OneStep Advanced Probe Kit (1 ml and 5 ml)

Product Description

TheQiagen QIAcuity OneStep Advanced Probe Kitis a high-performance reagent set designed for one-step reverse transcription digital PCR (RT-dPCR) on the QIAcuity Digital PCR System. Optimized for both RNA and RNA+DNA target detection, this kit simplifies workflows by combining reverse transcription and amplification into a single reaction. It supports singleplex and multiplex assays (up to 5-plex) and is compatible with QIAcuity Nanoplates, enabling precise quantification of low-abundance targets such as viral RNA or mRNA. Key components include a 4 PCR Master Mix, a 100 RT Mix, Enhancer GC for GC-rich targets, and an optional internal control RNA for assay validation.

Note: The price mentioned above is for reference only. Do not consider it for purchase. For the actual price and more product details, please contact us at +91 98508 70860, +91 84461 74263.

• HotStart RT Enzyme: Prevents off-target cDNA synthesis at room temperature, allowing for reaction setup and Nanoplate loading without premature reverse transcription.

• 4 Concentrated Master Mix: Facilitates higher sample loading per well, enhancing sensitivity and throughput.

• Advanced Inhibitor Tolerance: Demonstrates robust performance in the presence of common PCR inhibitors.

• Multiplex Capability: Supports detection of up to five targets in a single reaction using hydrolysis probes.

• Internal Control RNA: Includes QuantiNova Internal Control RNA for optional assay validation.

• Clinical Research: Quantification of viral RNA (e.g., SARS-CoV-2) and mRNA expression analysis.

• Environmental Monitoring: Detection of microbial RNA in wastewater and environmental samples.

• Biotechnology: Gene expression studies and RNA-based biomarker discovery.

• Pharmaceutical Development: Evaluation of RNA-based therapeutics and vaccine candidates

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