The Type-it Fast SNP Probe PCR Kit from QIAGEN features HotStarTaq Plus DNA Polymerase, which is inactive at room temperature to prevent non-specific amplification and primer-dimer formation. Activation occurs with a 5-minute incubation at 95C. The kit includes Q-Bond technology in the buffer, reducing annealing time to just 5 seconds and ensuring efficient amplification across various temperatures and magnesium concentrations. It also contains Q-Solution to support amplification of GC-rich regions, and ROX reference dye for consistent fluorescence normalization across real-time PCR instruments.
Fast Cycling Protocol: The inclusion of Q-Bond technology reduces PCR run times by up to 40%, enhancing throughput without sacrificing data quality.
Robust Performance on Difficult Templates: Effectively genotypes challenging SNP loci, including GC-rich regions, and performs reliably with as little as 1 ng of template DNA.
Clear Allelic Discrimination: Provides tight clustering and wide separation of allele signals, facilitating automated and accurate allele calling.
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