TheQiagen QIAcuity OneStep Advanced Probe Kitis a highperformance reagent set designed for onestep reverse transcription digital PCR RTdPCR on the QIAcuity Digital PCR System Optimized for both RNA and RNADNA target detection this kit simplifies workflows by combining reverse transcription and amplification into a single reaction It supports singleplex and multiplex assays up to 5plex and is compatible with QIAcuity Nanoplates enabling precise quantification of lowabundance targets such as viral RNA or mRNA Key components include a 4 PCR Master Mix a 100 RT Mix Enhancer GC for GCrich targets and an optional internal control RNA for assay validation
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Key Features
HotStart RT Enzyme Prevents offtarget cDNA synthesis at room temperature allowing for reaction setup and Nanoplate loading without premature reverse transcription
4 Concentrated Master Mix Facilitates higher sample loading per well enhancing sensitivity and throughput
Advanced Inhibitor Tolerance Demonstrates robust performance in the presence of common PCR inhibitors
Multiplex Capability Supports detection of up to five targets in a single reaction using hydrolysis probes
Internal Control RNA Includes QuantiNova Internal Control RNA for optional assay validation
Industries Served
Clinical Research Quantification of viral RNA eg SARSCoV2 and mRNA expression analysis
Environmental Monitoring Detection of microbial RNA in wastewater and environmental samples
Biotechnology Gene expression studies and RNAbased biomarker discovery
Pharmaceutical Development Evaluation of RNAbased therapeutics and vaccine candidates
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